报告时间：2012年10月30日（星期二）上午9:00 - 9:45

报告地点：生物楼学术报告厅

报告人：王子豪 博士

Sr. Research Scientist II, BioAnalytics, Grifols Inc,

Research Triangle Park, NC, USA

报告人简介：

Dr. Zihao Wang received his MS in bioinformatics from Michigan Technological University in 2003 and Ph.D. in Biological Chemistry in 2008 from the Johns Hopkins University School of Medicine in 2008. As his thesis work, Dr. Wang investigated both global and site-specific dynamics of the complex crosstalk between protein post-translational modifications by O-GlcNAc and O-phosphate using high-throughput mass spectrometry. Dr. Wang also developed and optimized a series of novel techniques for enrichment and identification of O-GlcNAc modification. He participated or led several research projects to study the potential roles of GlcNAcylation in regulating transcription, translation, and kinase activities. Dr. Wang's work was published in multiple premium scientific journals including PNAS, Science Signaling, Circulation Research, Diabetes, Molecular and Cellular Proteomics. After graduation, Dr. Wang spent a few months working as a Faculty Reserach Associate at JHU and then jointed Grifols Inc, a global pharmaceutical company specialized in providing plasma-derived therapeutics, as a Research Scientist and is now a Senior Research Scientist II and Manager of the Mass Spectrometry Laboratory. At Grifols, Dr. Wang led the extended molecular characterization of two recombinant therapeutic proteins and was a major contributor of one IND (Investigational New Drug) filing. He is a subject matter expert of mass spectrometry, liquid chromatography, glycomics, and general biochemical signal transduction.

报告摘要：

Protein post-translational modification by O-linked β-N-acetylglucosamine (O-GlcNAc) is abundant and dynamically regulated. O-GlcNAc is involved in almost all aspects of cellular functions, including transcription, translation, protein degradation, cell cycle, and stress responses. Accumulating evidence has suggested O-GlcNAc serves as a nutrient/stress sensor. Detection of O-GlcNAc by mass spectrometry remained extremely difficult due to lack of effective enrichment methods and the intrinsic labile nature of O-GlcNAc in gas phase. Here, development of a series of analytical methods that allow efficient and highly specific O-GlcNAc detection and quantification will be presented. Application of these analytical methods for studying the biological roles of O-GlcNAcylation and its implications in human diseases will also be discussed.

报告联系人：DNL2003组 赵旭（9396）