Single Molecule and Nanoparticle Imaging--in Biophysical, Surface, and Photocatalysis Studies

 

报告时间:2013年5月13日(星期一)14:00

报告地点:能源楼会议中心二号会议室(2楼204室)

报告人:Dr. NING FANG

Ames Laboratory, U.S. Department of Energy and Iowa State University 

报告提要:

●Rotational Tracking:The knowledge of rotational dynamics in and on live cells remains highly limited due to technical limitations. The single particle orientation and rotational tracking (SPORT) technique has been developed for visualizing rotational motions of anisotropic plasmonic gold nanorods under a differential interference contrast (DIC) microscope. The SPORT technique is capable of extracting important information (including rotational rates, modes, and directions) on the characteristic rotational dynamics involved in complex biological processes, such as endocytosis and intracellular transport.

●Fluorescence Super-Resolution and High-Precision Tracking:A fully automatic calibration and scanning prism-type total internal reflection fluorescence (TIRF) and scattering (TIRS) microscope has been constructed for high-precision tracking of single molecules and nanoparticles. Stable signals from non-blinking quantum dots under the automated TIRF microscope allows for continuous super-localization of single quantum dots with sub-10-nm precision in both lateral and axial directions. This system is also being used to reveal the nature and photocatalytic properties of the surface active sites on single Au-CdS hybrid nanocatalysts.

报告人简介:

Dr. Fang joined the faculty in August of 2008 as an Assistant Professor. He received his B.S. from Xiamen University, China in 1998 and his Ph.D. from the University of British Columbia, Canada in 2006 under Prof. David D.Y. Chen and was a Postdoctoral Associate at Iowa State University and Ames Laboratory, U.S. Department of Energy with Prof. Edward S. Yeung from 2006 to 2008.

近期论文:

? Nat. Commun. 2012, 3:1030.

? Chem. Rev. 2013, 113, 2469?2527.

? Nano Lett. 2013, 13(3), 1245-1250.

? Nano Lett. 2012, 12(8), 4282-4288.

? J. Am. Chem. Soc. 2012, 134(14), 6108-6111.

? Angew. Chem. Int. Ed. 2012, 51(31), 7734-7738.

? J. Am. Chem. Soc. 2011, 133(15), 5720-5723.

? J. Am. Chem. Soc. 2010, 132 (46), 16417-16422.

报告联系人:纳米生物分析和效应研究组(18T1)吴仁安(9828)

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