报告时间：2013年9月27日（星期五）上午9:30-10:30 

　　报告地点：催化基础国家重点实验室三楼会议室

　　报告人：Dr. Paul D. Ashby 

　　Lawrence Berkeley National Laboratory, USA 

　　报告摘要： 

　　Membrane protein clustering is important for many signaling processes and cellular function.  Resolving proteins within clusters with both high temporal and spatial resolution has been challenging for both optical and atomic force microscopy. The challenge for atomic force microscopy has been deformation of the sample due to the minimum force of imaging and mechanical limitations of the scanners to scan quickly. I will present innovations from my group to address both of these issues. I will discuss different AFM control theories and why reducing interaction force is best accomplished through cantilever innovation. Then, I will present my groups efforts with both nanowire cantilevers and encased cantilevers, the latter of which have 10 times higher sensitivity than regular cantilever in solution. Lastly, I will discuss a new scanning mode we developed and how it enables much higher scan speeds.   

　　报告人简介： 

　　EDUCATION 

　　1996 - 2003               Harvard University, MA 

　　Ph.D. thesis Intermolecular and Interfacial Forces: Elucidating Molecular Mechanisms using Chemical Force Microscopy, with Professor Charles M. Lieber Department of Chemistry and Chemical Biology. 

　　1992 - 1996               Westmont College, CA 

　　B.S. in Chemistry  Magna Cum Laude  

　　RESEARCH EXPERIENCE  

　　2008-present              Lawrence Berkeley National Lab, CA 

　　Staff Scientist – Image materials and interfaces in solution with high spatial and temporal resolution using novel scanned probe microscopy techniques and tools.   

　　2004-2007           Lawrence Berkeley National Lab, CA 

　　Postdoctoral Fellow – Implemented various scanned probe techniques to investigate water structure near well defined chemical surfaces, surface potential of protein surfaces, viscoelastic characteristics of novel nanocrystal superlattices, and magnetic and electrical properties of spintronic devices.  

　　2003-2004           Massachusetts Institute of Technology, MA 

　　Postdoctoral Fellow – Developed novel microelectro-mechanical systems (MEMS) for separation, concentration, and label-free detection of biological molecules.  

　　1997-2003           Harvard University, MA 

　　Research Assistant – Illuminated the molecular origin of materials properties by measuring intermolecular and interfacial forces using the Atomic Force Microscope.   

　　1996-1997           Harvard University, MA 

　　Research Assistant - Studied Bacteriorhodopsin conformational dynamics using ultrafast spectroscopy.  

　　1994-1996           Westmont College, CA 

　　Research Assistant - Measured Energy Transfer in doped 2-indanone using ODMR.  

　　FUNDED PROPOSALS  

　　2008 Molecular Foundry Director’s Fund Membrane protein and cell wall organization and dynamics using chemically modified Fiber Force Probes. 

　　2009 NSF CBET-0940417 Real-time adaptive imaging algorithms for atomic force microscopy with Andrea Bertozzi(UCLA) and Jim DeYoreo(LBL). 

　　2009 Molecular Foundry Challenge Grant Imaging neuronal axons and synapses at 10-nm resolution with Bruce Cohen(LBL), P. James Schuck(LBL), and Stefano Cabrini(LBL). 

　　2010 Intel Research Grant Toward 16nm lithography: a multifaceted approach to discovery and characterization of collapse-free resist with Deirdre Olynick (LBL) and James Blackwell (Intel).