报告时间：2015年3月11日（星期三）上午9：30
报告地点：生物楼406会议室
报告人：张会 教授 （美国约翰霍普金斯大学）
报告人简介：
Education and Training (in chronological order):
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1989
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B.S. Plant Biochemistry, Beijing University, Beijing, China.
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1992
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M.S. Gene and Protein Engineering, Beijing University, Beijing, China.
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1999
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PhD Biochemistry, University of Pennsylvania, Philadelphia, PA.
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Professional Experience (in chronological order):
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1998-1999
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Product Manager, New England Biolabs, Beverly, MA.
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1999-2001
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Scientist and Senior Scientist, Cell Signaling Technology, Beverly, MA.
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2001-2003
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Research Scientist, Institute for Systems Biology, Seattle, WA.
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2003-2006
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Senior Research Scientist, Institute for Systems Biology, Seattle, WA.
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2006-2011
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Assistant Professor, Department of Pathology, Johns Hopkins University, Baltimore, MD.
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2011-present
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Associate Professor, Department of Pathology, Johns Hopkins University, Baltimore, MD.
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2012-present
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Director of Mass Spectrometry Core Facility, Center for Biomarker Discovery and Translation. Johns Hopkins University, Baltimore, MD
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报告摘要：
Protein glycosylation is one of the most common forms of protein modifications. Each glycoprotein can be glycosylated at different glycosites and each glycosite may be modified by different glycans. This structural heterogeneity provides additional functions for each glycoprotein in physiological and pathological processes. However, the structural heterogeneity also complicates the studies of structure-function relationships of glycoproteins. To rapidly identify and quantify the glycosylation on each glycosite from complex biological mixtures and to understand the function of protein glycosylation, we developed an integrated approach for global proteomics, glycoproteomics, and glycomics, with which peptides and glycans are isolated and separated by liquid-chromatography followed by quantitative analysis by mass spectrometry. When we performed integrated quantitative analyses to determine protein abundance, glycosylation site occupancy and glycan structures, we showed by specific examples that upon the identification of glycoproteins, glycosites containing specific glycans were readily identifiable and quantifiable. The applications of integrated approach to biological problems facilitate the understanding of glycosylated forms in cellular functions.
联系人：1809组 叶明亮 （9620）
